Journal article
Y. Sato, Lennart Hilbert, H. Oda, Yinan Wan, John M. Heddleston, T. Chew, V. Zaburdaev, Philipp J. Keller, T. Lionnet, N. Vastenhouw, H. Kimura
Development, 2019
Development, 2019
DOI:
10.1242/dev.179127
DOI
PubMed
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APA
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Sato, Y., Hilbert, L., Oda, H., Wan, Y., Heddleston, J. M., Chew, T., … Kimura, H. (2019). Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis. Development. https://doi.org/10.1242/dev.179127
Chicago/Turabian
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Sato, Y., Lennart Hilbert, H. Oda, Yinan Wan, John M. Heddleston, T. Chew, V. Zaburdaev, et al. “Histone H3K27 Acetylation Precedes Active Transcription during Zebrafish Zygotic Genome Activation as Revealed by Live-Cell Analysis.” Development (2019).
MLA
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Sato, Y., et al. “Histone H3K27 Acetylation Precedes Active Transcription during Zebrafish Zygotic Genome Activation as Revealed by Live-Cell Analysis.” Development, 2019, doi:10.1242/dev.179127.
BibTeX Click to copy
@article{y2019a,
title = {Histone H3K27 acetylation precedes active transcription during zebrafish zygotic genome activation as revealed by live-cell analysis},
year = {2019},
journal = {Development},
doi = {10.1242/dev.179127},
author = {Sato, Y. and Hilbert, Lennart and Oda, H. and Wan, Yinan and Heddleston, John M. and Chew, T. and Zaburdaev, V. and Keller, Philipp J. and Lionnet, T. and Vastenhouw, N. and Kimura, H.}
}
Abstract
ABSTRACT Histone post-translational modifications are key gene expression regulators, but their rapid dynamics during development remain difficult to capture. We applied a Fab-based live endogenous modification labeling technique to monitor the changes in histone modification levels during zygotic genome activation (ZGA) in living zebrafish embryos. Among various histone modifications, H3 Lys27 acetylation (H3K27ac) exhibited most drastic changes, accumulating in two nuclear foci in the 64- to 1k-cell-stage embryos. The elongating form of RNA polymerase II, which is phosphorylated at Ser2 in heptad repeats within the C-terminal domain (RNAP2 Ser2ph), and miR-430 transcripts were also concentrated in foci closely associated with H3K27ac. When treated with α-amanitin to inhibit transcription or JQ-1 to inhibit binding of acetyl-reader proteins, H3K27ac foci still appeared but RNAP2 Ser2ph and miR-430 morpholino were not concentrated in foci, suggesting that H3K27ac precedes active transcription during ZGA. We anticipate that the method presented here could be applied to a variety of developmental processes in any model and non-model organisms. Summary: FabLEM, an endogenous labeling technique that uses modification-specific antigen-binding fragments, is used to examine changes in histone modification levels and transcription during zygotic genome activation in live zebrafish embryos.